Summary
Type: SPE
Application #: 305530
Catalog#: 730012 - SPE Cartridges, Chromabond, C18 ec, 3ml, PP, 200mg, 50/pk
Detection:
Conditions: Column conditioning: 2 x 2 mL methanol, then 2 x 2 mL water
(Do not let run the column dry.)
Sample aspiration: prepared sample is passed through the column by vacuum
Washing: 3 mL water
Drying: nitrogen
Elution: 1.5 mL dichloromethane, then 1.0 mL dichloromethane – 2-propanol – 25 % ammonia (8:2:0.2, v/v/v)
Evaporation: to dryness (50 °C, gentle stream of nitrogen)
Reconstitution: 50 µL acetonitrile
Authors: Pietsch, J. et al.
Source: J. Sep. Sci. 2008, 31, 2410–2416